Introduction To SB Carlsberg Flask Yeast propagation

The SB Carlsberg Flask is filled to its net capacity with wort, corresponding to approximately 80percent of the total volume. Sterilization takes place using an autoclave, a gas burner or an electric hotplate. It is then placed in a refrigerator or a cold room to cool the wort to the desired working temperature. The cold wort is aerated through the membrane sample valve connected to the aeration lance. Yeast culture can be introduced aseptically through the membrane fitting by means of a syringe. Alternatively, dry yeast culture can be transferred to the flask through the empty filter housing.